EUROIMMUN provides reliable, state-of-the art in vitro test systems for the determination of specific IgE antibodies in patient sera With “ CCD band ” as an additional indicator for the clinical relevance of Speci?c IgE results.

In the EUROLINE test system membrane strips with 20 different allergens are used, allowing a detailed patient allergy profile to be obtained with a single test. Moreover, the specific antibodies are determined semi quantitatively by comparing the intensities with the help of “ Euroline Scan System ”

Profiles Available...

1. Euroline India Specific Food Profile
2. Euroline India Specific Inhalation Profile
3. Euroline Allergy Profile Atopy
4. Euroline Allergy Profile Pediatrics
5. Euroline Allergy Profile Insect Venoms

Key Features

  Separate Membrane strip for each sample

  Highly pure antigens: No cross-reaction

  Easy and reliable differentiation between positive and negative samples

  Intensity of the antigen bands correlate with the antibody titer

  Automated semi-quantitative evaluation with EUROLineScan

  Classification of results in RAST/EAST system

  Excellent correlation with the Phadia CAP system

  Small sample volumes (only 100 µl), ideally suited for Paediatrics

  Detection of specifc IgE antibodies against Cross-reactive Carbohydrate Determinants (CCD) on India specific Food      and Inhalation profiles.

  Fast test performance – approx. 2.5 hours with only 100 µl sample

India Specific Food

  Milk

  Hen eggs yolk

  Milkpowder

  Wheat Flour

  Rice

  Soyabean

  Peanuts

  Coconut

  Apple

  Grape

  Potato

  Spinach

  Onion

  Cucumber

  Chicken

  Mustard

  Coffee

  Ginger

  Crab

  Prawn

  CCD

  Indicator

India Specific Inhalation

  Timothy grass

  Rye cultivated

  Corn

  Eucalyptus sp.

  Parthenium hysterophorus

  Carnation flower

  Sunflower seeds

  Mites(D1 & D2)

  Dermatophagoides farinae

  Cockroach

  Cat epithelium

  Dog hair

  Pigeon feathers

  Chicken feathers

  Aspergillus fumigatus

  Tricophyton Mentagrophytes

  Cotton yarn and fabric

  Straw dust

  Jute

  Sheeps wool

  CCD

  Indicator

CCD

Many allergens are Glycoproteins containing oligosaccharide side chains which are bound to the protein framework of these allergens. In some cases, patients develop specific antibodies against these carbohydrate structures. The abbreviation CCD stands for “Cross-reactive Carbohydrate Determinant”. CCDs can be found in a large number of allergens of vegetable or animal origin. Due to their significant similarity in structure, CCDs are known to cause a strong cross reactivity.
Although the importance of specific IgE antibodies against CCDs has not yet been fully understood, they are considered to be irrelevant for diagnosis in most cases and as such complicate the interpretation of positive in vitro diagnostic results. For this reason, the presence of specific IgE antibodies against CCDs may provide useful additional information, especially with positive IgE results that disagree with the clinical picture, and can serve as an interpretation aid in the evaluation of overall test results. The determination of specific IgE antibodies against CCDs can be useful in the following cases:

  Clinical symptoms disagreeing with the specific IgE result obtained (e.g. in positive serological results for peanuts)

  In many positive specific IgE results for plant foods

  Sensitization of persons suffering from pollen allergies to CCD-rich latex without work-related exposition

  Detection of specific IgE antibodies against honey bee or wasp venom without clinical symptoms

EUROLINE SCAN

The Programme EUROLine Scan has been developed by EUROIMMUN to allow fully automated, quantitative evaluation of membrane-based test systems, to facilitate administration of extensive individual data and to provide detailed documentation of results.
The incubated membrane strips are scanned onto a protocol sheet using a flatbed scanner. EURO Line Scan recognizes the position of the strips, even if they have been laid inexactly. It then identifies the bands, measures their intensity and automatically converts them into EAST (Enzyme Allergo Sorbent Test) classes, which correlate to the concentrations given in the well-known RAST (Radio Allergo Sorbent Test) system. The computer-based evaluation can be monitored and the data modified manually, whereby all changes are automatically documented. The results are then saved together with the image data. It is no longer necessary to archive incubated test strips. A separate result sheet can be produced for each patient.